| 1. | Chapter 3 sequencing cdna fragments of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) partial clones were sequenced which were selected from the differentially expressed clones screened by cdna macroarray analysis
3中华绒螯蟹卵巢发育相关基因cdna片段的序列测定从cdna微阵列筛选的差异表达克隆中选取部分克隆进行测序。 |
| 2. | Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method , then the inserted fragments in plasmids were amplified by pcr
2用cdna微阵列筛选中华绒螯蟹卵巢发育相关基因将差减cdna文库中的所有克隆用碱裂解法提取质粒，然后以提取的质粒为模板，用pcr方法扩增插入片段。 |
| 3. | Chapter 2 cloning and analysis of the full - length cdna of ejo1 gene related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) primers were designed according to the sequence of est004 ( dbest accen - ssion number : ca591895 ) which was gotten using ssh and cdna macroarray approach
3中华城螫蟹卵巢发育相关基因ej03的全长0 a克隆和序列分析根据ssh结合cdna微阵列技术获得的est003 （ dbest检索号： ca591894 ）的序列设计引物，用5 ’ race和3 ’ race的方法获得了ejo3基因的全长cdna序列。 |
| 4. | Part 1 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of subtractive cdna library of mitten crab ( eriocheir japonica sinensis ) ovary two subtracted cdna libraries of mitten crab ( eriocheir japonica sinensis ) ovaries at two successive developmental stages were constructed by suppression subtractive hybridization ( ssh )
第一部分中华绒螯蟹卵巢发育相关基因的筛选1中华绒螯蟹卵巢差减cdna文库的构建应用抑制性差减杂交技术，构建了中华绒螯蟹卵巢两个发育时期的差减cdna文库。 |
| 5. | Part 2 cloning and analysis of the full - length cdnas of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of race cdna library of mitten crab ( eriocheir japonica sinensis ) ovary in order to get full - length cdna sequences of the differentially expressed genes , a race cdna library of the mitten crab ovary at stage iii was constructed successfully with smart technique
南京师范大学博士研究生毕业论文第二部分中华绒螫蟹卵巢发育相关基因全长叨a的克隆及序列分析工中华绒蟹蟹卵巢ra kc a文库的构建为了获得差异表达基因的全长cdna序列，运用smart技术，构建了中华绒蟹蟹卵巢（ ill期） racecdna文库。 |
